https://hdl.handle.net/20.500.12809/265 2026-04-17T11:05:50Z https://hdl.handle.net/20.500.12809/10670 Apoptotic and antiproliferative effects of Inula viscosa L. water extract in the expression of microRnas on HCT 116 cell line: an in vitro study Yıldırım, Betül Apaydın; Gedikli, Semin); Kordalı, Şaban; Küçükaydın, Selçuk In this study, the antiproliferative and apoptotic effects of Inula viscosa L. water extract (IVE) on HCT 116 has been examined, and the change in the expression of miRNAs. Phenolic compounds of IVE were determined as mu g/g extract using by HPLC-DAD. Quantitative determination of apoptosis, cell viability, IC50 values and miRNAs of the cells were determined during 24, and 48 hours. IVE contain coumarin, rosmarinic acid and chlorogenic acid. According to the findings of our study, the expression of miR-21 and miR-135a1 was upregulated, and miR-145 was downregulated in HCT 116 cells (Control). Additionally, IVE was found to have significant potential in regulating miRNAs, downregulating miR-21, miR-31 and miR-135a1, and upregulating miR-145 in HCT-116 cells. All these results show that the anticancer effect of IVE via regulating miRNAs' expression has been demonstrated for the first time, and may be candidate biomarkers in colorectal cancer. 2023-01-01T00:00:00Z https://hdl.handle.net/20.500.12809/10386 Insecticidal activities of some plant essential oils on Rhyzopertha dominica (F.) and Sitophilus granarius L. (Coleoptera: Bostrichidae and Curculionidae) adults Kordali, Şaban; Kesdek, Memiş; Akkan, Ümran; Usanmaz Bozhöyük, Ayşe In this study, the insecticidal activities of the essential oils extracted from Cuminum cyminum L., Mentha longifolia L. and Allivum sativum L. by hydrodistillation method using the Clevenger apparatus were investigated against Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) and Sitophilus granarius L. (Coleoptera: Curculionidae) adults. The tests were carried out under 25±1°C temperature, 65±5 proportional humidity and 16:8 (light:dark) conditions and doses of 5, 10, 15, 20 μL/petri. The results of the tested essential oils showed that they had insecticidal effect at different rates (between 1.25% and 100%) 96 hours after exposure. Among the essential oils tested, it was determined that the oil of A. sativum showed the highest effect against R. dominica and S. granarius adults, while the essential oils of C. cyminum and M. longifolia showed the lowest effect against each two pests. Additionally, LC50 and LC90 values of these oils against R. dominica and S. granarius adults were seperately recorded. According to LC50 and LC90 values, it was reported that M. longifolia and C. cyminum essential oils were more effective against adults of R. dominica (0.698 μL/insect, LC50 and 4.694, LC90) and S. granarius (0.131 μL/ insect, LC50 and 2.452 LC90), respectively. These datas showed that the mortalities increased depending on the increase in the doses of the tested three plant essential oils. Most importantly, considering all these results, it is seen that these essential oils can be used as a natural and environmentally friendly biopreparate against harmful R. dominica and S. granarius adults in stored grain crops.; Bu çalışmada, Clevenger düzeneği kullanılarak, hidrodistilasyon yöntemiyle elde edilen Cuminum cyminum L., Mentha longifolia L. ve Allivum sativum L. uçucu yağlarının ekin kambur biti, Rhyzopertha dominica (F.) ve buğday biti, Sitophilus granarius L. (Coleoptera: Bostrichidae ve Curculionidae) erginlerine karşı insektisidal aktiviteleri araştırılmıştır. Testler 25±1°C sıcaklık, 65±5 orantılı nem ve 16:8 (aydınlık:karanlık) şartlarda ve 5, 10, 15, 20 μL/petri dozlarında yapılmıştır. Sonuçlar, bu uçucu yağların uygulamadan 96 saat sonra farklı oranlarda (%1.25 ile %100 arasında) insektisidal etkiye sahip olduklarını göstermiştir. Test edilen uçucu yağlardan A. sativum’un yağının R. dominica ve S. granarius erginlerine karşı en yüksek oranlarda etki gösterdiği, bununla birlikte C. cyminum ve M. longifolia uçucu yağlarının ise bu iki zararlıya karşı en düşük etki gösterdiği saptanmıştır. Ayrıca, bu uçucu yağların, R. dominica ve S. granarius erginlerine karşı LC50 ve LC90 değerleri de kaydedilmiştir. LC50 ve LC90 değerlerine göre, R. dominica (0.698 μL/böcek, LC50 ve 4.694, LC90) ve S. granarius (0.131 μL/böcek, LC50 ve 2.452 LC90) erginlerine karşı, M. longifolia ve C. cyminum uçucu yağlarının daha etkili oldukları kaydedilmiştir. Bu sonuçlar, test edilen üç bitki uçucu yağlarının dozların artışına bağlı olarak ölümlerin de arttığını göstermiştir. Daha da önemlisi, bütün bu sonuçlar dikkate alındığında bu uçucu yağların depolanmış tahıl ürünlerinde zararlı R. dominica ve S. granarius erginlerine karşı doğal ve çevre dostu biyopraperat olarak kullanılabileceği görülmektedir. 2022-01-01T00:00:00Z https://hdl.handle.net/20.500.12809/10359 Phytotoxic Effects of Three Origanum Species Extracts and Essential Oil on Seed Germinations and Seedling Growths of Four Weed Species Kordali, Şaban; Kabaağaç, Gülbahar; Şen, İsmail; Yılmaz, Ferah; Najda, Agnieszka The use of chemical pesticides to protect agricultural products is a global concern because of their adverse effects on the environment and public health. To avoid the dangers of synthetic herbicides, research has turned to natural alternatives. This study was conducted to evaluate the allelopathic effect of essential oil (EO) extracted from Origanum syriacum, Origanum onites, and Origanum majorana. In addition, the chemical composition of the essential oil was elucidated by gas chromatography and mass spectrometry (GC–MS) analysis. A total of 11 different components of O. syriacum were identified, and the main components were carvacrol (88.49), p-Cymene (5.71), γ-Terpinene (1.63), β-Caryoplhyllene (1.48), and Terpinen-4-ol (0.65), respectively. For O. onites, 10 different compounds were identified, and the main components were carvacrol (58.65), Thymol (30.97), Linalool (4.17), p-Cymene (1.94), and β-Caryoplhyllene (0.98), respectively. Finally, for O. majorana, 14 different compounds were identified, and the main components were carvacrol (40.57), α-Terpineol (29.28), p-Cymene (9.02), γ-Terpinene (5.80), and carvacrol methyl ether (3.46). Finally, 14 compounds from the Origanum majorana species were identified, with carvacrol (40.57), -Terpineol (29.28), p-Cymene (9.02), and -Terpinene (5.80) as the parent compound (3.46). Oxygenated monoterpenes were the highest in all species’ EO content. EOs and plant extracts were tested at 5, 10, and 20 L/Petri concentrations against seed germination and seedling growth in four weed species (Thlaspi arvense, Amaranthus retroflexus, Rumex cripus, and Lactuca serriola). The concentrations of essential oil were set as 5, 10, and 20 µL/Petri dishes for seed germination. In the greenhouse experiment, the final concentration of solutions was set as 20 µL and the solutions were directly sprayed on the surface of the weeds, and the mortality rates were noted after 24 and 48 h of application. It was observed that increasing the application decreased seed germination. The phytotoxic effects on the seedling germination in the greenhouse were observed, resulting in 48.76–94% mortality rates. Consequently, the essential oil from Origanum species could be considered as an alternative bio-herbicide to tested weeds. 2022-01-01T00:00:00Z https://hdl.handle.net/20.500.12809/10218 Effects of boric acid and potassium metaborate on cytokine levels and redox stress parameters in a wound model infected with methicillin‑resistant Staphylococcus aureus Çelebi, Demet; Taghizadehghalehjoughi, Ali; Başer, Sümeyye; Genç, Sıdıka; Kordali, Şaban; Yılmaz, Ferah Methicillin‑resistant Staphylococcus aureus (MRSA) infections are usually found in hospital settings and, frequently, in patients with open wounds. One of the most critical virulence factors affecting the severity and recurrence of infections is the biofilm; increasing antibiotic resistance due to biofilm formation has led to the search for alternative compounds to antibiotics. The present study aimed to use boric acid and potassium metaborate against MRSA infection in a fibroblast wound model. For this purpose, a two‑part experiment was designed: First, MRSA strains were used for the test, and both boric acid and potassium metaborate were prepared in microdilution. In the second step, an MRSA wound model was prepared using a fibroblast culture, and treatments with boric acid and potassium metaborate were applied for 24 h. For the evaluation of the effects of treatment, cell viability assay (MTT assay), analysis of redox stress parameters, including total oxidant status and total antioxidant capacity analyses, lactate dehydrogenase analysis and immunohistochemical staining were performed. In addition, IL‑1β and IL‑10 gene expression levels were assayed. According to the results, potassium metaborate was more effective and exhibited a lower toxicity to fibroblast cells compared to boric acid; moreover, potassium metaborate decreased the level of prooxidant species and increased the antioxidant status more effectively than boric acid. The IL‑1β level in the bacteria group was high; however, boric acid and potassium metaborate significantly decreased the expression levels of inflammatory markers, exhibiting the potential to improve the resolution of the lesion. On the whole, the findings of the present study suggest that boric acid and potassium metaborate may be effective on the tested microorganisms 2022-01-01T00:00:00Z