Bitkisel Ve Hayvansal Üretim Bölümü Koleksiyonu

Determination of the wing morphology differentiation of old and recent honey bee samples from western Turkey using geometric morphometrics

Fri, 01 Jan 2021 00:00:00 GMT

Determination of the wing morphology differentiation of old and recent honey bee samples from western Turkey using geometric morphometrics Köseoğlu, Mustafa; İvgin Tunca, Rahşan; Özsoy, Neslihan; Tuna, Yahya Tuncay; Topal, Erkan; Günbey, Belgin In this study, old and recent honey bee, Apis mellifera L., 1758 (Hymenoptera: Apidae) specimens were compared using geometric morphometrics. The old honey bee samples were collected from different apiaries in Edirne, Balıkesir, Çanakkale, Denizli and Muğla Provinces, and Gökçeada (an Aegean island) in Turkey in 1987-1988 under a project of the Aegean Agricultural Research Institute and the recent samples were collected in the same locations in 2017. The mean values determined for each region were grouped using Mahalanobis distances, and the results were summarized on dendrogram. While the old samples constituted one group, the recent samples constituted another one. When the results of the discriminant function analysis were compared, it was observed that overall old and recent samples were statistically different from each other (P < 0.0001). The evaluation of both groups has revealed that the recent population of Gökçeada was different from morphologically the other mainland populations in the current situation. However, the Thrace (Edirne) honey bee specimens were different from the Anatolian (Çanakkale, Denizli, Balıkesir, Muğla) and island (Gökçeada) specimens in the past according to dendrogram relationships.; Bu çalışmada eski ve yeni bal arısı, Apis mellifera L., 1758 (Hymenoptera: Apidae) örnekleri geometrik morfometrik yöntemlerle morfolojik olarak karşılaştırılmıştır. Eski bal arısı örnekleri, Ege Tarımsal Araştırma Enstitüsü tarafından yürütülen bir proje kapsamında, 1987-1988 yıllarında Edirne, Balıkesir, Çanakkale, Denizli, Muğla illeri ve Gökçeada Adası'ndaki farklı arılıklardan toplanmıştır ve 2017 yılında aynı lokasyonlardan alınan güncel bal arısı örnekleri ile çalışılmıştır. Her bölge için belirlenen ortalama değerler Mahalanobis mesafesi kullanılarak gruplandırılmış ve sonuçlar dendrogram üzerinde özetlenmiştir. Eski örnekler bir grubu oluştururken, güncel örnekler bir diğerini oluşturmuştur. Eski ve güncel örnekler için diskriminant fonksiyon analizi sonuçları karşılaştırıldığında, geçmiş ve şimdiki genel örneklerin istatistiksel olarak birbirinden farklı olduğu gözlenmiştir (P < 0.0001). Her iki grubun değerlendirmesi, Ada'nın (Gökçeada) mevcut popülasyonunun, mevcut durumda diğer anakara popülasyonlarından morfolojik olarak farklı olduğunu ortaya koymuştur. Öte yandan Trakya (Edirne) bal arısı popülasyonunun, dendrograma göre geçmişte Anadolu (Çanakkale, Denizli, Balıkesir, Muğla) ve Ada (Gökçeada) bal arısı örneklerinden farklı olduğu belirlenmiştir

MICROSCOPIC AND MOLECULAR DETECTION OF NOSEMA SP. IN THE SOUTHWEST AEGEAN REGION

Fri, 01 Jan 2021 00:00:00 GMT

MICROSCOPIC AND MOLECULAR DETECTION OF NOSEMA SP. IN THE SOUTHWEST AEGEAN REGION Kartal, Serengül; İvgin Tunca, Rahşan; Özgül, Okan; Karabaǧ, Kemal; Koç, Hasan Beekeeping, performed in many parts of the world, has a very large place in the world trade market with bee products such as wax, bee venom, propolis and royal jelly, especially honey production. However, honey bee diseases are quite common and restricted the production of bee products. One of the most important of these diseases, Nosema, is caused by spores in intestinal epithelium cells of the honeybee. Nosema apis and Nosema ceranae are the factors of this disease and also common in our country. These two species can be distinguished from each other by molecular diagnostic methods. In this study, materials collected from 152 apiaries located in 13 districts of Mugla province and 62 water sources close to these apiaries. The spores were counted using Thoma lame under light microscope. DNA isolation was carried out from spore positive samples. 218MITOC FOR-REV and 321APIS FOR-REV primers were used to figure out the N. apis and N. ceranae species. After DNA sequence analysis of the obtained amplifications, it was determined that all samples formed 3 haplotypes according to studied sequences for the first time. In Mugla region, the presence of only N. ceranae as a disease agent was determined and the prevalence of the disease was detected at a rate of 71.53±6.02%. Moreover, blast analysis showed that the N. ceranae sequence detected high similarity (94-100 %) with the previously reported in Lebanon, France, Morocco and Thailand samples.

SUCCESS OF SELECTION IN TERMS OF HYGIENIC BEHAVIOR IN STRUGGLE AGAINST DWV AND VARROA

Wed, 01 Jan 2020 00:00:00 GMT

SUCCESS OF SELECTION IN TERMS OF HYGIENIC BEHAVIOR IN STRUGGLE AGAINST DWV AND VARROA Karabağ, Kemal; Alemli, Ayşe; İvgin Tunca, Rahşan Many studies have reported sudden and large-scale losses in honey bee colonies. There is no precise explanation for this happening, but it may be caused by the interaction of pathogenic viruses and ectoparasite Varroa destructor. Deformed-wing virus (DWV) is common in Apis mellifera L. and has been implicated in worldwide Varroa-associated colony losses. Hygienic behavior may be one of the ways that limit V. destructor infestation level and DWV load. The purpose of this research was to determine whether the selection of higher hygienic behavior is effective against V. destructor and DWV. Workers and pupae of A. m. anatoliaca were collected from colonies showing four generations of hygienic behaviodr, and non-hygienics were used as a control. Loads of DWV were investigated by one-step RT-qPCR, and the bottom-board method was used to allow mites count. The prevalence of DWV in pupae were higher in hygienic colonies (69.0%, average viral load 234.38) than control colonies (60.8% average viral load 937.70). However, the prevalence of worker bees infected with DWV was lower in hygienic colonies (41.3%, average viral load 181627.69) than in control’s (66.0%, average viral load 241982.35). Although the averages of the Varroa counts were significantly different for both hygienic (28.92) and control colonies (108.90), we could not find any correlation between DWV loads of both workers and pupae in this study. When all these results are evaluated, the selection performed in terms of hygienic behavior can said to have been said successful against DWV.

New Records from Inner West Anatolia to the Turkish Limoniidae (Insecta, Diptera) Fauna

Fri, 01 Jan 2010 00:00:00 GMT

New Records from Inner West Anatolia to the Turkish Limoniidae (Insecta, Diptera) Fauna Özgül, Okan; Koç, Hasan In this study 4 new records belonging to Limoniidae are be found from Inner West Anatolia in 2009. Locations of 4 new records are given in this paper. These species are; Hoplolabis (Parilisia) pontica, Afrolimnophila minima, Austrolimnophila (Austrolimnophila) ochracea and Limnophila (Limnophila) schranki. The distribution of these 4 new records from Kutahya and Usak provinces are mapped and localities of this species are characterized. Also distributions of these species in Palearctic Region are given. WOS: 000285897000006