Electrochemical Determination of Hemoglobin by the İmmobilization of the Analyte into a Carbon Felt Electrode (CFE) Using Cyclic Voltammetry (CV)

Abstract

A practical and sensitive electrochemical sensor for hemoglobin (Hb) detection was developed. The main electrochemical reaction was the reduction of Fe (III) in the Hb structure. For the construction of the sensor, Hb was physically immobilized onto a carbon felt electrode (CFE) without using any linker or applying any pre-functioning or electrochemical procedures. After examining the electrochemical mechanism, the experimental conditions, such as pH, were optimized and the analytical characteristics were investigated. A linear range of 0.05 µM to 3.00 µM with a detection limit of 4.4 nM was obtained for Hb. The relative standard deviation value for 3 replicate determinations of 0.05 µM Hb was calculated as1.35%. The developed sensor was successfully applied for Hb detection via standard addition method in synthetic physiological serum sample. Meanwhile, the influences of interfering reagents, such as cysteine, ascorbic acid, bovine serum albumin, and glucose, on Hb detection were also examined.