Purification and Characterization of Laccase from Thermophilic Anoxybacillus Gonensis P39 and its Application of Removal Textile Dyes

Abstract

Textile dyes which are extensively used have important incomes commercially however they cause health and environmental problems. In this study, Anoxybacillus gonensis P39 (Gen Bank No: FJ808725) isolated from Erzurum-Ilica Spring and laccase from A. gonensis P39 was purified as 160 kDa molecular weight by using (NH4)(2)SO4 precipitation, DEAE-cellulose and Sephacryl S200 coloumn with 4.16, 11.96 and 83.4 purification folds, orderly. Vmax and Km were calculated as 0.442 mu mol/min. mg and 0.077 mM. Optimum pH and temperature were 5.0 and 60 degrees C moreover, laccase was stable against high temperature and pH conditions. Although most of the metal ions were positively affected laccase activity, Mn2+, Cu2+ and Fe2+ were strong inhibitors. Consequently, removal of some textile dyes was evaluated and the laccase reduced the Reactive Black5, Fuchsine, Allura Red and Acid Red 37 dyes by 22.1, 5.96, 1.34 and 1.15%, respectively so that A. gonensis P39 can be successfully used for bioremediation of waste water.