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dc.contributor.authorDemir, Nazan
dc.contributor.authorDemir, Yasar
dc.contributor.authorCoskun, Fatma
dc.contributor.authorTasgin, Esen
dc.date.accessioned2020-11-20T16:22:26Z
dc.date.available2020-11-20T16:22:26Z
dc.date.issued2012
dc.identifier.issn0970-7077
dc.identifier.urihttps://hdl.handle.net/20.500.12809/4149
dc.descriptionWOS: 000300464200023en_US
dc.description.abstractCarbonic anhydrase is an enzyme that takes responsibility in inhalation function but, until today, carbonic anhydrase is not examined if it is present in the bovine brain membrane or not. The enzyme carbonic anhydrase was purified and separately characterized according to the bonding forms in 4 steps such as outer peripheral, cytosolic, inner peripheral and integral. Affinity chromatography was used for purification of the enzyme in all four steps. The affinity column was prepared with sepharose-4B-L-tyrosine-sulphanilamide. Purified carbonic anhydrase was obtained at each step. Enzyme activity was measured by CO2 hydratase activity and esterase activity methods. Optimum pH and optimum temperature were defined for purified enzymes at each step. Morover molecular weight and purity were detected by gel filtration and SDS-PAGE electrophorose. In addition, the enzyme's Km and v(max) values were found with the Lineweaver-Burk method. The obtained results are discussed in comparison with other mammalian carbonic anhydrases. Carbonic anhydrase was shown to be exist in bovine brain membrane and this enzyme was-optimized.en_US
dc.item-language.isoengen_US
dc.publisherAsian Journal of Chemistryen_US
dc.item-rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBrain Membraneen_US
dc.subjectCarbonic Anhydraseen_US
dc.titleExpression of Cytosolic and Noncytosolic Carbonic Anhydrase Enzymes from Bovine Brain Membraneen_US
dc.item-typearticleen_US
dc.contributor.departmenten_US
dc.contributor.departmentTemp[Demir, Nazan; Demir, Yasar] Mugla Univ, Dept Chem, Fac Sci, Mugla, Turkey -- [Coskun, Fatma] Ataturk Univ, Dept Chem, Fac Sci, TR-25240 Erzurum, Turkey -- [Tasgin, Esen] Bayburt Univ, Bayburt Educ Fac, TR-69100 Bayburt, Turkeyen_US
dc.identifier.volume24en_US
dc.identifier.issue4en_US
dc.identifier.startpage1511en_US
dc.identifier.endpage1514en_US
dc.relation.journalAsian Journal of Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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