The Antibacterial Activities of Lavandula stoechas and Crepis sancta Leaf and Flower Against Mastitis Pathogens and Enzymatic and NonEnzymatic Antioxidant Activities of the Extracts

Abstract

The antibacterial and antioxidant activities of Lavandula stoechas and Crepis sancta extracts on subclinical mastitis bacteria in cows have not been reported to the present day. The aim of this work was to investigate of the antibacterial and antioxidant potential of methanol extract from L. stoechas and C. sancta. Methanol extracts were screened for antibacterial activity against mastitis pathogens. The antibacterial activity was tested using disc diffusion and broth dilution method. In addition, the plant extracts were tested against the stable DPPH (1,1-Diphenyl-2-picrylhydrazyl) free-radical. Additionally methanol extracts were screened for enzymatic antioxidant activity. The methanol extract of C. sancta showed maximum inhibition zone against S. aureus-18 (16 mm). MIC (minimum inhibition concentration) value was determined as 3250 µg/mL for test bacteria. The highest radical scavenging capacity (62 %) was obtained from the flower extracts of L. stoechas and the leaf extracts of C. sancta. The methanol extract of C. sancta displayed a strong antioxidant activity (trolox equivalent = 1.6 mM/g). It was determined that the activities of catalase (CAT) and ascorbate peroxidase (AP) enzymes were higher at the flowers of L. stoechas compared to the leaves. The extracts of L. stoechas and C. sancta leaf and flower have antibacterial and antioxidant potential against subclinic mastitis causative agent Staphylococci species.