DETERMINATION OF BIOACTIVE COMPONENTS AND ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACTS OF MISTLETOE LEAVES (VISCUM ALBUM L. SUBSP. ALBUM L.)

Abstract

The extract of ethanol, methanol, hexane, chloroform, isopropanol and water of mistletoe leaves (Viscum album L. subsp. album L.) were tested for antimicrobial activity against 11 bacteria and one yeast by disc diffusion method. In our study, methanolic extracts mistletoe of leaves showed the best inhibition zones against Streptococcus faecalis and Bacillus subtilis (22mm). Inhibition zones of the all of the extracts varied 6 to 22 mm against tested microorganisms. MICs of V. album subsp. album different extracts obtained by the broth serial dilution method, showed the lowest sensitivity to S.faecalis and B.subtilis with 4mg/ml concentration of methanolic extracts. Methanolic extracts of mistletoe were among the most active with the MIC values ranging from 4-256 mg/mL.